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KMID : 1160220010290010007
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Mycobiology
2001 Volume.29 No. 1 p.7 ~ p.10
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PCR-Based Sensitive Detection of Wood-Decaying Fungus Phellinus linteus by Specific Primer from rDNA ITS Regions
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Park Dong-Suk
Kang Hee-Wan
Kim Ki-Tae
Cho Soo-Muk
Park Young-Jin
Shin Hye-Sun
Lee Byoung-Moo
Go Seung-Joo
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Abstract
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Based on the rDNA ITS sequences data, specific primer set for PCR detection of wood-decaying fungus Phellinus linteus was designed. The length of PCR products using designed primer set(SHF and SHR) was about 540 bp. Among 11 species, 17 isolates of Phellinus spp. including Phellinus linteus, P. pomaceus, P. spiculosus, P. baumi, P. pini, P. igniarius, P. gilvus, P. biscuspidatus, P. weirii, P. johnsonianus, P. robutus, and P. igniarius, seven isolates of Phellinus linteus showed about 540 bp-sized single band. This molecular technique could offer a useful tool for detecting and identifying Phellinus linteus.
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KEYWORD
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Phellinus linteus, rDNA, Primer, Detection
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