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KMID : 1160220010290040179
Mycobiology
2001 Volume.29 No. 4 p.179 ~ p.182
Cloning and Phylogenetic Analysis of Chitin Synthase Genes from Tricholoma matsutake
Suh Seok-Jong

Kim Il-Hyeon
Nam Ju-Hyun
Ghim Sa-Youl
Bae Kyung-Sook
Kim Jong-Guk
Abstract
Chitin synthases(UDP-N-acetyl-D-glucosamine: chitin 4-¥â-N-acetyl-D-glucosaminyl transferase, EC 2.4.1.16) catalyze the synthesis of chitin from UDP-N-acetyl-D-glucosamine. Two zymogenic type of chitin synthase gene(TmCHS1 and TmCHS2) were amplified and its nucleotide sequences were determined. By the amino acid comparison and UPGMA tree grouping, TmChs1 and TmChs2 were classified as class II and class IV chitin synthases respectively. The class II type TmChs1 was grouped with others of Agaricales ectomycorrhizal mushroom. Additionally the phylogenetic tree was well adapted to Hymenomycete previously classified by morphological and physiological characteristics.
KEYWORD
Tricholoma matsutake, Chitin synthase
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