|
KMID : 1160220010290040183
|
|
Mycobiology
2001 Volume.29 No. 4 p.183 ~ p.189
|
|
|
Characteristics of the Amylase and its Related Enzymes Produced by Ectomycorrhizal Fungus Tricholoma matsutake
|
|
Hur Tae-Chul
Ka Kang-Hyun
Joo Sung-Hyun
Terashita Takao
|
|
|
Abstract
|
|
|
|
Extracellular amylase properties were examined with the mycelium of Tricholoma matsutake isolated from ectomycorrhizal roots of Pinus densiflora. The molecular weights of α-amylase and glucoamylase were estimated as 34.2 kD and 11.5 kD, respectively, after eluted through Superdex 75 column. The optimum pH of the purified enzyme was found in a range of pH 5.0~6.0, with a peak at pH 5.0. The activities of these enzymes were stable from 4oC to 30oC. The α-amylase of T. matsutake readily hydrolyzed soluble starch and amylose-B, while it weakly hydrolyzed glycogen, dextrin, amylose and amylose-A. The main products of hydrolysis were confirmed to be glucose, maltose and maltotriose on the basis of the similarities in the thin layer chromatographic mobility.
|
|
|
KEYWORD
|
|
|
α-Amylase, Glucoamylase, Tricholoma matsutake
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|