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KMID : 1160220020300010005
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Mycobiology
2002 Volume.30 No. 1 p.5 ~ p.12
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Mycoflora of Chicken-Viscera with Aid of RAPD Technique as a Tool for Confirmation
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Gherbawy Youssuf A.
Farghaly Refaat M.
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Abstract
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A total of 100 samples of chicken-viscera were collected from different poultry-slaughtering houses in Austria;(20 samples of each of gizzard, heart, intestine, liver and spleen). Intestine and gizzard were heavy contaminated with moulds than other examined visceral organs(4.4×105 and 2.6×104 colonies/1g of the samples, respectively). Fungal contamination was not detected in all samples of heart and spleen. Eighty-five mould isolates were collected from the examined samples, the majority of isolates belonging to Aspergillus glaucus group(20.0%) and Trichoderma(14.1%). These isolates comprised 15 species belonging to 9 genera. Members of Aspergillus glaucus(telomorph: Eurotium) group and Trichoderma were further confirmed their identification using random amplified polymorphic DNA-polymerase chain reaction(RAPD-PCR) technique.
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KEYWORD
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Chicken, Viscera, Mould, RAPD-PCR
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