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KMID : 1160220020300010022
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Mycobiology
2002 Volume.30 No. 1 p.22 ~ p.26
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Physiological Characteristics of Symbiotic Fungi Associated with the Seed Germination of Gastrodia elata
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Hong In-Pyo
Kim Han-Kyoung
Park Jeong-Sik
Kim Gwang-Po
Lee Min-Woong
Guo Shun-Xing
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Abstract
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This study was carried out to investigate the possibility for seeds germination of Gastrodia elata using symbiotic fungi. Since seeds of G. elata are very small and lack an endosperm and other nutrients, their germination is difficult without requirement for external nutrients. Out of twenty six isolates collected from protocorms of G. elata and roots of native orchids inhabited in wild, two strains(H-2 and H-21) were observed to stimulate the seed germination of G. elata. The seed germination of G. elates was excellent on oak tree leaves medium. The optimal conditions for mycelial growth of symbiotic fungi were 25oC and pH 6.0, respectively. The mycelial growth of H-2 strain was excellent on YMA medium, while H-21 was poor on PDA medium. In case of carbon sources, the mycelial growth of H-2 and H-21 was good on media supplemented with glucose and dextrin, respectively. Calcium nitrate was good for mycelial growth of H-2 strain as a nitrogen sources, whereas urea was effective to H-21 strain.
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KEYWORD
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Gastrodia elata, Mycelial growth, Seeds germination
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