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KMID : 1160220020300020088
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Mycobiology
2002 Volume.30 No. 2 p.88 ~ p.92
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Isolation and Characterization of the Salicylic Acid Induced Gene in Rehmannia glutinosa by Differential Display
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Kim Hee-Jong
Kim Kwon-Jong
Lee Youn-Su
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Abstract
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Rehmannia glutinosa is a perennial medicinal plant belonging to the family Scrophulariaceae with more than 300 species known in the world, especially in temperate regions. Its roots have been used widely in Korea for medicinal purposes. However, it is commonly infected by various pathogens during storage, causing great damage to the roots, and impedes the intensive farming of the crop. Therefore, an attempt has been made to isolate and screen a resistance gene against the pathogen Fusarium oxysporum using differential display. We treated salicylic acid(SA), and isolated a resistance gene that responds to SA. As a result, we found that SA was involved in plant defense mechanism in pathogenicity tests with SA treated and non-treted plants, and we isolated a partial PR-la gene through differential display polymerase chain reaction(DD-PCR) method.
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KEYWORD
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Differential display, Fusarium oxysporum, PR1-a gene, Rehmannia glutinosa, Salicylic acid
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