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KMID : 1160220020300040202
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Mycobiology
2002 Volume.30 No. 4 p.202 ~ p.207
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PCR Based Detection of Phellinus linteus using Specific Primers Generated from Universal Rice Primer(URP) Derived PCR Polymorphic Band
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Kang Hee-Wan
Park Dong-Suk
Park Young-Jin
Lee Byoung-Moo
Cho Soo-Muk
Kim Ki-Tae
Seo Geon-Sik
Go Seung-Joo
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Abstract
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This study was carried out to develop specific primers for PCR detection of Phellinus linteus. Diverse genomes of 15 Phellinus spp. including five Phellinus linteus isolates were fingerprinted by Primer Universal rice primer(URP)1F. The URP-PCR pattern differentiated P. linteus isolates from other phellinus spp. A polymorphic band(2.8 kb), which is unique for P. linteus isolates, was isolated and sequenced. Twenty four-oligonucleotide primer pairs were designed based on information of DNA sequence. The primer set(PLSPF2/PLSPR1) amplified single band(2.2 kb) of expected size with genomic DNA from seven Phellinus linteus, but not with that of other Phellinus species tested. The primers could be used identically in both DNA samples from mycelium and fruit bodies. This specific primers could offer a useful tool for detecting and identifying P. linteus rapidly.
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KEYWORD
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PCR polymorphic band, Phellinus linteus, Specific primers, URP primer
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