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KMID : 1160220030310010042
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Mycobiology
2003 Volume.31 No. 1 p.42 ~ p.45
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Transformation of the Edible Basidiomycete, Pleurotus ostreatus to Phleomycin Resistance
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Kim Beom-Gi
Joh Jung-Ho
Yoo Young-Bok
Magae Yumi
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Abstract
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For transformation of Pleurotus ostreatus, two novel vectors, pPhKM1 and pPhKM2, were constructed, using the regulatory sequences of the P. sajor-caju β-tubulin gene(TUB1) and the ble gene encoding phleomycin binding protein. pPhKM1 contains ble fused to the TUB1 promoter and the Schizophyllum commune GPD terminator. pPhKM2 contains ble fused to the promoter and terminator regions of P. sajor-caju TUB1. To confirm phleomycin-resistance activity, each vector was cotrans-formed with pTRura3-2 into the P. ostreatus homokaryotic ura? strain. The transforming DNA was stably integrated into the genomic DNA. Subsequently, phleomycin resistance was conferred on wild-type dikaryotic P. ostreatus by transformation with pPhKM1 or pPhKM2. This transformation system generated stable phleomycin-resistant transformants.
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KEYWORD
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Basidiomycete, Phleomycin resistance, Pleurotus ostreatus, Transformation, β-tublin
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