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KMID : 1160220050330020121
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Mycobiology
2005 Volume.33 No. 2 p.121 ~ p.123
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Immobilization of Keratinase from Aspergillus flavus K-03 for Degradation of Feather Keratin
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Kim Jeong-Dong
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Abstract
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Extracellular keratinase isolated from Aspergillus flavus K-03 was immobilized on calcium alginate. The properties and reaction activities of free and immobilized keratinase with calcium alginate were characterized. The immobilized keratinase showed proteolytic activity against soluble azo-casein and azo-keratin, and insoluble feather keratin. Heat stability and pH tolerance of keratinase were greatly enhanced by immobilization. It also displayed a higher level of heat stability and an increased tolerance toward alkaline pHs compared with free keratinase. During the durability test at 40oC, 48% of the original enzyme activity of the immobilized keratinase was remained after 7 days of incubation. The immobilized keratinase exhibited better stability, thus increasing its potential for use in industrial application.
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KEYWORD
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Durability, Heat stability, Immobilization, Keratinase, pH tolerance
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