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KMID : 1160220080360030173
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Mycobiology
2008 Volume.36 No. 3 p.173 ~ p.177
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Antishigellosis and Cytotoxic Potency of Crude Extracts and Isolated Constituents from Duranta repens
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Nikkon Farjana
Habib M. Rowshanul
Karim M. Rezaul
Hossain M. Shamim
Mosaddik M. Ashik
Haque M. Ekramul
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Abstract
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The crude ethanol extracts (stem and fruits), their fractions and two triterpenes, β-Amyrin and 12-Oleanene 3β, 21β-diol, isolated as a mixture from the chloroform soluble fraction of an ethanolic extract of Duranta repens stem, were evaluated for antibacterial, antifungal activities by the disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The structures of the two compounds were confirmed by IR, 1H-NMR, 13C-NMR and LC-MS spectral data. The chloroform soluble fraction of stem and ethanol extract of fruits possess potent antishigellosis activity and also exhibited moderate activity against some pathogenic bacteria and fungi but the isolated compound 1 (mixture of β-Amyrin and 12-Oleanene 3β, 21βdiol) showed mild to moderate inhibitory activity to microbial growth. The minimum inhibitory concentrations (MICs) of the extracts (stem and fruits), their fractions and compound 1 were found to be in the range of 32~128 μg/ml. The chloroform soluble fractions of stem and ethanol extract of fruit showed significant cytotoxicity with LC50 value of 0.94 μg/ml and 0.49 μg/ml, respectively against brine shrimp larvae.
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KEYWORD
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β-Amyrin, Antimicrobial activity, Cytotoxicity, Duranta repens, 12-Oleanene-3-β, 21-β-diol
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