KMID : 1160220080360030178
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Mycobiology 2008 Volume.36 No. 3 p.178 ~ p.182
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Submerged Culture of Phellinus linteus for Mass Production of Polysaccharides
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Lee June-Woo
Baek Seong-Jin Kim Yong-Seok
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Abstract
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In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients
of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; K2HPO4, 0.46 g/l; KH2PO4, 1.00 g/l; MgSO4¡¤7H2O, 0.50 g/l; FeCl2¡¤62O, 0.01 g/l; MnCl2¡¤4H2O, 0.036 g/l; ZnCl2, 0.03 g/l; and CuSO4¡¤7H2O, 0.005 g/l. The optimal culture conditions were determined to be as follows: temperature, 28oC; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively. Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.
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KEYWORD
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Cultural condition, Optimal media, Phellinus linteus, Polysaccharide
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