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KMID : 1160220080360030178
Mycobiology
2008 Volume.36 No. 3 p.178 ~ p.182
Submerged Culture of Phellinus linteus for Mass Production of Polysaccharides
Lee June-Woo

Baek Seong-Jin
Kim Yong-Seok
Abstract
In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients
of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; K2HPO4, 0.46 g/l; KH2PO4, 1.00 g/l; MgSO4¡¤7H2O, 0.50 g/l; FeCl2¡¤62O, 0.01 g/l; MnCl2¡¤4H2O, 0.036 g/l; ZnCl2, 0.03 g/l; and CuSO4¡¤7H2O, 0.005 g/l. The optimal culture conditions were determined to be as follows: temperature, 28oC; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively. Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.
KEYWORD
Cultural condition, Optimal media, Phellinus linteus, Polysaccharide
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