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KMID : 1160220090370010031
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Mycobiology
2009 Volume.37 No. 1 p.31 ~ p.36
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Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhydrosophoradiol- 3-acetate Isolated from Calotropis gigantea (Linn.) Flower
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Habib M. Rowshanul
Karim M. Rezaul
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Abstract
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A phytochemical study on the flowe r of Calotropis gigantea (Linn.) using silica gel column chromatography and preparative thin layer chromatography, led to the first time isolation of Di-(2-ethylhexyl) phthalate (compound 1) and anhydrosophoradiol-3-acetate (compound 2). The structures of these compounds were confirmed by spectroscopic analyses (IR, HRTOFMS and NMR). The antibacterial and antifungal activities of ethyl acetate extract, compound 1 and compound 2 were measured using the disc diffusion method. Ethyl acetate extract and compound 1 presented better results than compound 2. The minimum inhibitory concentrations (MICs) of the extract and compounds were found to be in the range of 16~128 μg/ml. The cytotoxicity (LC50) against brine shrimp nauplii (Artemia salina) were also evaluated and found to be 14.61 μg/ml for ethyl acetate, 9.19 μg/ml for compound 1 and 15.55 μg/ml for compound 2.
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KEYWORD
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Anhydrosophoradiol-3-acetate, Antimicrobial, Calotropis gigantea and Di-(2-ethylhexyl) phthalate
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