KMID : 1160220090370010062
|
|
Mycobiology 2009 Volume.37 No. 1 p.62 ~ p.66
|
|
Occurrence of Internal Stipe Necrosis of Cultivated Mushrooms (Agaricus bisporus) Caused by Ewingella americana in Korea
|
|
Lee Chan-Jung
Jhune Chang-Sung Cheong Jong-Chun Yun Hyung-Sik Cho Weon-Dae
|
|
Abstract
|
|
|
The internal stipe necrosis of cultivated mushrooms (Agaricus bisporus) is caused by the bacterium Ewingella americana,a species of the Enterobacteriaceae. Recently, Ewingella americana was isolated from cultivated white button mushrooms inKorea evidencing symptoms of internal stipe browning. Its symptoms are visible only at harvest, and appear as a variablebrowning reaction in the center of the stipes. From these lesions, we isolated one bacterial strain (designated CH4). Inoculationof the bacterial isolate into mushroom sporocarps yielded the characteristic browning symptoms that were distinguishablefrom those of the bacterial soft rot that is well known to mushroom growers. The results of Gram stain, flagellalstaining, and biochemical tests identified these isolates as E. americana. This was verified by pathogenicity, physiological andbiochemical characteristics, and the results of an analysis of the 16S rRNA gene sequences and the fatty acids profile. Thisis the first report of the isolation of E. americana from cultivated white button mushrooms in Korea.
|
|
KEYWORD
|
|
Agaricus bisporus, Ewingella americana, Internal stipe necrosis, Mushroom, 16S rRNA gene
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|