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KMID : 1160220090370040251
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Mycobiology
2009 Volume.37 No. 4 p.251 ~ p.257
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Identification and Characterization of Eurotium rubrum Isolated from Mejuin Korea
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Yun Yeo-Hong
Hyun Min-Woo
Suh Dong-Yeon
Kim Yong-Min
Kim Seong-Hwan
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Abstract
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We isolated and identified a strain of Eurotium rubrum from Meju that has not been reported in Korea. This fungus isyellowish brown; reverse dark brown on CYA and PDA while yellow on 2% MEA at 25oC. Cleistothecia are first brightyellow and gradually turned brown. Mycerial growth on CYA attained a diameter of 30 mm at 20oC, 37 mm at 25oC and32 mm at 30oC after 15 days. The isolate grew slower on 2% MEA (< 20 mm 15 days at 25oC) compared to CYA and PDA(< 40 mm 15 days at 25oC). Cleistothecia are superficial, yellow to light brown, globose to subglobose, 40~75 μm in diameter. Asci are 8-spored and globose to subglobose 8~11 μm. Ascospores are disciform, 4.0~5.0 μm in length and 4.2~4.5 μm inwidth. Conidia are ovate or bacillar, finely roughened to densely spinulose, 4.6~6.0 μm in length and 3.0~4.3 μm in width. Compared to known Eurotium rubrum, the Korean isolate showed 99% sequence similarity in ITS rDNA (554 bp) and calmodulin(750 bp) gene and 100% in β-tubulin (1016 bp) gene. The E. rubrum isolate also had weak β-glucosidase and proteaseactivities
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KEYWORD
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β-tubulin gene, Calmodulin gene, Eurotium rubrum, ITS rDNA, Meju
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