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KMID : 1160220090370040313
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Mycobiology
2009 Volume.37 No. 4 p.313 ~ p.316
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Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum
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Jo Woo-Sik
Bae Soon-Hwa
Cho Doo-Hyun
Park So-Deuk
Yoo Young-Bok
Park Seung-Chun
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Abstract
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To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35oC. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at 25oC for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum.
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KEYWORD
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Cellulolytic enzyme, Chromogenic media, Congo red, Ganoderma lucidum
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