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KMID : 1160220090370040317
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Mycobiology
2009 Volume.37 No. 4 p.317 ~ p.319
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Development of Specific Primer for Tricholoma matsutake
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Kim Jang-Han
Han Yeong-Hwan
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Abstract
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In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'- GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.
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KEYWORD
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ITS, Tricholoma matsutake, Tricholomataceae
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