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KMID : 1160220100380010026
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Mycobiology
2010 Volume.38 No. 1 p.26 ~ p.32
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Functional Characterization of cAMP-Regulated Gene, CAR1, in Cryptococcus neoformans
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Jung Kwang-Woo
Maeng Shin-Ae
Bahn Yong-Sun
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Abstract
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The cyclic AMP (cAMP) pathway plays a major role in growth, sexual differentiation, and virulence factor synthesis of pathogenic fungi. In Cryptococcus neoformans, perturbation of the cAMP pathway, such as a deletion in the gene encoding adenylyl cyclase (CAC1), causes defects in the production of virulence factors, including capsule and melanin production,as well as mating. Previously, we performed a comparative transcriptome analysis of the Ras- and cAMP- pathway mutants,which revealed 163 potential cAMP-regulated genes (38 genes at a 2-fold cutoff). The present study characterized the role of one of the cAMP pathway-dependent genes (serotype A identification number CNAG_ 06576.2). The expression patterns were confirmed by Northern blot analysis and the gene was designated cAMP-regulated gene 1 (CAR1). Interestingly, deletion of CAR1 did not affect biosynthesis of any virulence factors and the mating process, unlike the cAMP-signaling deficient cac1Δ mutant. Furthermore, the car1Δ mutant exhibited wild-type levels of the stress-response phenotype against diverse environmental cues, indicating that Car1, albeit regulated by the cAMP-pathway, is not essential to confer a cAMP-dependent phenotype in C. neoformans.
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KEYWORD
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CAR1, Cryptococcus neoformans, Cyclic AMP, Transcriptome analysis
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