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KMID : 1160220100380010070
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Mycobiology
2010 Volume.38 No. 1 p.70 ~ p.73
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Screening of Cell Cycle-Related Genes of Pleurotus eryngii Using Yeast Mutant Strains
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Shi Shanliang
Ro Hyeon-Su
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Abstract
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Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over 30oC. Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from 30oC to 37oC. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined.
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KEYWORD
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Cell cycle, Genomic DNA library, Pleurotus eryngii, Temperature sensitive, Yeast
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