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KMID : 1160220100380010074
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Mycobiology
2010 Volume.38 No. 1 p.74 ~ p.77
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Development of Detection Methods for Cellulolytic Activity of Auricularia auriculajudae
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Jo Woo-Sik
Bae Soon-Hwa
Choi Seung-Yong
Park So-Deuk
Yoo Young-Bok
Park Seung-Chun
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Abstract
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To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent,pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35oC. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25oC for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
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KEYWORD
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Auricularia auricula-judae, Cellulolytic enzyme, Chromogenic media, Congo red
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