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KMID : 1160220100380020108
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Mycobiology
2010 Volume.38 No. 2 p.108 ~ p.112
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Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Pro-teome Analysis
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Cho Soo-Jin
Kim Young-Hwan
Park Hee-Moon
Shin Kwang-Soo
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Abstract
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To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations,6 proteins were up-regulated in the lkh1+ null mutant and 4 proteins successfully identified: glycolipid anchored surface precursor, β-glucosidase (Psu1), cell surface protein, glucan 1,3-β-glucosidase (Bgl2), and exo-1,3 β-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.
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KEYWORD
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Lkh1, Proteome analyses, Schizosaccharomyces pombe
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