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KMID : 1160220100380020128
Mycobiology
2010 Volume.38 No. 2 p.128 ~ p.132
Fruit Body Formation on Silkworm by Cordyceps militaris
Hong In-Pyo

Kang Pil-Don
Kim Ki-Young
Nam Sung-Hee
Lee Man-Young
Choi Yong-Soo
Kim Nam-Suk
Kim Hye-Kyung
Lee Kwang-Gill
Humber Richard A.
Abstract
Injection inoculation protocols for fruit body formation of Cordyceps militaris (C. militaris) were investigated to improve the incidence of infection in the silkworm species Bombyx mori (B. mori). Injection, with suspensions of C. militaris hyphal bodies into living silkworm pupae, was used to test for fruit body production. Use of Daeseungjam rather than Baegokjam or Keumokjam varieties of B. mori is thought to be suitable for infection by C. militaris. From mounting, nine-day-old to 11-day-old pupae showed the best incidence of infection with a 100 ¥ìL injection volume. Silkworm pupae injected with a hyphal suspension concentration of more than 2 ¡¿ 105 colony-forming unit (cfu) recorded a greater than 96% incidence of infection. Also, fruit bodies of C. militaris were induced and produced at a light intensity between 500 and 1,000 lx.
KEYWORD
Bombyx mori, Cordyceps militaris, Fruit body, Hyphal bodies, Injection inoculation
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