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KMID : 1160220100380020137
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Mycobiology
2010 Volume.38 No. 2 p.137 ~ p.143
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Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide
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Pham Tuan Anh
Kim Jeong-Jun
Kim Keun
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Abstract
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The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, 25oC culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran,2% corn steep powder medium, initial conidia concentration of 107 conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization.
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KEYWORD
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Beauveria bassiana, Conidia production, Optimal culture conditions, Solid-state fermentation
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