|
KMID : 1160220110390010020
|
|
Mycobiology
2011 Volume.39 No. 1 p.20 ~ p.25
|
|
|
Improvement of Fungal Cellulase Production by Mutation and Optimization of Solid State Fermentation
|
|
Vu Van Hanh
Pham Tuan Anh
Kim Keun
|
|
|
Abstract
|
|
|
|
Spores of Aspergillus sp. SU14 were treated repeatedly and sequentially with Co60 γ-rays, ultraviolet irradiation, and Nmethyl-N'-nitro-N-nitrosoguanidine. One selected mutant strain, Aspergillus sp. SU14-M15, produced cellulase in a yield 2.2-fold exceeding that of the wild type. Optimal conditions for the production of cellulase by the mutant fungal strain using solid-state fermentation were examined. The medium consisted of wheat-bran supplemented with 1% (w/w) urea or NH4Cl,1% (w/w) rice starch, 2.5mM MgCl2, and 0.05% (v/w) Tween 80. Optimal moisture content and initial pH was 50% (v/w)and 3.5, respectively, and optimal aeration area was 3/100 (inoculated wheat bran/container). The medium was inoculated with 25% 48 hr seeding culture and fermented at 35oC for 3 days. The resulting cellulase yield was 8.5-fold more than that of the wild type strain grown on the basal wheat bran medium.
|
|
|
KEYWORD
|
|
|
Aspergillus sp., Cellulase, Mutation, Optimization of solid state fermentation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|