KMID : 1160220110390010020
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Mycobiology 2011 Volume.39 No. 1 p.20 ~ p.25
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Improvement of Fungal Cellulase Production by Mutation and Optimization of Solid State Fermentation
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Vu Van Hanh
Pham Tuan Anh Kim Keun
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Abstract
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Spores of Aspergillus sp. SU14 were treated repeatedly and sequentially with Co60 ¥ã-rays, ultraviolet irradiation, and Nmethyl-N'-nitro-N-nitrosoguanidine. One selected mutant strain, Aspergillus sp. SU14-M15, produced cellulase in a yield 2.2-fold exceeding that of the wild type. Optimal conditions for the production of cellulase by the mutant fungal strain using solid-state fermentation were examined. The medium consisted of wheat-bran supplemented with 1% (w/w) urea or NH4Cl,1% (w/w) rice starch, 2.5mM MgCl2, and 0.05% (v/w) Tween 80. Optimal moisture content and initial pH was 50% (v/w)and 3.5, respectively, and optimal aeration area was 3/100 (inoculated wheat bran/container). The medium was inoculated with 25% 48 hr seeding culture and fermented at 35oC for 3 days. The resulting cellulase yield was 8.5-fold more than that of the wild type strain grown on the basal wheat bran medium.
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KEYWORD
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Aspergillus sp., Cellulase, Mutation, Optimization of solid state fermentation
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