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KMID : 1160220110390010064
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Mycobiology
2011 Volume.39 No. 1 p.64 ~ p.66
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Enhanced Expression of Glucose 2-Oxidase in Phlebia tremellosa by Addition of Phthalates
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Kim Baik-Joong
Kim Hye-won
Choi Hyoung-T.
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Abstract
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Most fungi possess several hydrogen peroxide-generating enzymes, glucose oxidase and pyranose oxidase. Pyranose oxidase can use glucose as its substrate to generate hydrogen peroxide. White rot fungi, which degrade diverse recalcitrant compounds,contain lignin-degrading enzymes, and lignin peroxidase and manganese peroxidase require hydrogen peroxide for their enzymatic reactions. In this study, we isolated a cDNA fragment of pyranose oxidase from Phlebia tremellosa using PCR and examined its expression under the degradation conditions of diethylphthalate (DEP). Pyranose oxidase expression was enhanced up to 30% by the addition of DEP, and this result supports the possible involvement of pyranose oxidase in the degradation of recalcitrant compounds.
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KEYWORD
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Hydrogen peroxide generation, Phlebia tremellosa, Pyranose oxidase
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