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KMID : 1160220110390020071
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Mycobiology
2011 Volume.39 No. 2 p.71 ~ p.78
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Multi-loci Molecular Characterisation of Endophytic Fungi Isolated from Five Medicinal Plants of Meghalaya, India
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Bhagobaty Ranjan Kumar
Joshi S. R.
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Abstract
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The phylogenetic relationships of the most dominant and morphologically cryptic endophytic fungal isolates from each of five selected medicinal plants, namely Potentilla fulgens, Osbeckia stellata, Osbeckia chinensis, Camellia caduca, and Schima khasiana of the biodiversity rich state of Meghalaya, were assessed with random amplification of polymorphic DNA and PCR-restriction fragment length polymorphism profiles. Sequencing of the internal transcribed spacer 1, small subunit rRNA and partial β-tubulin gene fragments was also conducted to determine the phylogenetic relationships of these isolates with fungal sequences available in Genbank, NCBI. The identity of the fungal isolates is suggested based on the molecular phylogenetic data.
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KEYWORD
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Cryptic morphology, Endophytic fungi, Identification, Medicinal plants, Molecular phylogeny
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