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KMID : 1160220110390020092
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Mycobiology
2011 Volume.39 No. 2 p.92 ~ p.95
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Optimal Culture Conditions for Mycelial Growth of Lignosus rhinocerus
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Lai W. H.
Murni M. J. Siti
Fauzi D.
Mazni O. Abas
Saleh N. M.
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Abstract
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Lignosus rhinocerus is a macrofungus that belongs to Polyporaceae and is native to tropical regions. This highly priced mushroom has been used as folk medicine to treat diseases by indigenous people. As a preliminary study to develop a culture method for edible mushrooms, the cultural characteristics of L. rhinocerus were investigated in a range of culture media under different environmental conditions. Mycelial growth of this mushroom was compared on culture media composed of various carbon and nitrogen sources in addition to C/N ratios. The optimal conditions for mycelial growth were 30oC at pH 6 and 7. Rapid mycelial growth of L. rhinocerus was observed on glucose-peptone and yeast extract peptone dextrose media. Carbon and nitrogen sources promoting mycelial growth of L. rhinocerus were glucose and potassium nitrate, respectively. The optimum C/N ratio was approximately 10 : 1 using 2% glucose supplemented as a carbon source in the basal media.
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KEYWORD
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Cultural conditions, Edible mushroom, Lignosus rhinocerus, Medicinal mushroom, Mycelial growth
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