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KMID : 1160220120400040258
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Mycobiology
2012 Volume.40 No. 4 p.258 ~ p.262
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Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
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Son Yu-Lim
Kim Hyoun-Young
Thiyagarajan Saravanakumar
Xu Jing Jing
Park Seung-Moon
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Abstract
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cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 μM within 90 min.
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KEYWORD
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Dye decolorization, Glyoxal oxidase, Manganese peroxidase, Phanerochaete chrysosporium, Pichia pastoris
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