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KMID : 1160220130410030149
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Mycobiology
2013 Volume.41 No. 3 p.149 ~ p.154
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Production and Characterization of a New α-Glucosidase Inhibitory Peptide from Aspergillus oryzae N159-1
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Kang Min-Gu
Yi Sung-Hun
Lee Jong-Soo
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Abstract
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An α-glucosidase inhibitor was developed from Aspergillus oryzae N159-1, which was screened from traditional fermented Korean foods. The intracellular concentration of the inhibitor reached its highest level when the fungus was cultured in tryptic soy broth medium at 27oC for five days. The inhibitor was purified using a series of purification steps involving ultrafiltration, Sephadex G-25 gel permeation chromatography, strong cation exchange solid phase extraction, reverse-phase high performance liquid chromatography, and size exclusion chromatography. The final yield of the purification was 1.9%. Results of the liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicated that the purified α-glucosidase inhibitor was a tri-peptide, Pro-Phe-Pro, with the molecular weight of 360.1 Da. The IC50 value of the peptide against α-glucosidase activity was 3.1 mg/mL. Using Lineweaver-Burk plot analysis, the inhibition pattern indicated that the inhibitor acts as a mixed type inhibitor.
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KEYWORD
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α-Glucosidase inhibitory peptide, Anti-obesity, Aspergillus oryzae N159-1
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