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KMID : 1160220130410040252
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Mycobiology
2013 Volume.41 No. 4 p.252 ~ p.255
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Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers
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Kim Sang-Woo
Kim Sin-Il
Lee Hyun-Jun
Park Ju-Wan
Ro Hyeon-Su
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Abstract
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Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.
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KEYWORD
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Fungal pathogens, ITS primer, Mushroom, Pleurotus eryngii
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