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KMID : 1160220140420010034
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Mycobiology
2014 Volume.42 No. 1 p.34 ~ p.40
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Three New Non-reducing Polyketide Synthase Genes from the Lichen-Forming Fungus Usnea longissima
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Wang Yi
Wang Juan
Cheong Yong-Hwa
Hur Jae-Seoun
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Abstract
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Usnea longissima has a long history of use as a traditional medicine. Several bioactive compounds, primarily belongingto the polyketide family, have been isolated from U. longissima. However, the genes for the biosynthesis of these compounds areyet to be identified. In the present study, three different types of non-reducing polyketide synthases (UlPKS2, UlPKS4, andUlPKS6) were identified from a cultured lichen-forming fungus of U. longissima. Phylogenetic analysis of product templatedomains showed that UlPKS2 and UlPKS4 belong to group IV, which includes the non-reducing polyketide synthases with anmethyltransferase (MeT) domain that are involved in methylorcinol-based compound synthesis; UlPKS6 was found to belong togroup I, which includes the non-reducing polyketide synthases that synthesize single aromatic ring polyketides, such as orsellinicacid. Reverse transcriptase-PCR analysis demonstrated that UlPKS2 and UlPKS4 were upregulated by sucrose; UlPKS6 wasdownregulated by asparagine, glycine, and alanine.
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KEYWORD
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Fungal non-reducing polyketide synthase, Lichen forming-fungi, mRNA expression, Usnea longissima
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