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KMID : 1160220140420010052
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Mycobiology
2014 Volume.42 No. 1 p.52 ~ p.58
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A Nudix Hydrolase Protein, Ysa1, Regulates Oxidative Stress Response and Antifungal Drug Susceptibility in Cryptococcus neoformans
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Lee Kyung-Tae
Kwon Hyo-Jeong
Lee Do-Hyun
Bahn Yong-Sun
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Abstract
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A nucleoside diphosphate-linked moiety X (Nudix) hydrolase-like gene, YSA1, has been identified as one of thegromwell plant extract-responsive genes in Cryptococcus neoformans. Ysa1 is known to control intracellular concentrations ofADP-ribose or O-acetyl-ADP-ribose, and has diverse biological functions, including the response to oxidative stress in theascomycete yeast, Saccharomyces cerevisiae. In this study, we characterized the role of YSA1 in the stress response and adaptationof the basidiomycete yeast, C. neoformans. We constructed three independent deletion mutants for YSA1, and analyzed theirmutant phenotypes. We found that ysa1 mutants did not show increased sensitivity to reactive oxygen species-producingoxidative damage agents, such as hydrogen peroxide and menadione, but exhibited increased sensitivity to diamide, which is athiol-specific oxidant. Ysa1 was dispensable for the response to most environmental stresses, such as genotoxic, osmotic, andendoplasmic reticulum stress. In conclusion, modulation of YSA1 may regulate the cellular response and adaptation of C. neoformans to certain oxidative stresses and contribute to the evolution of antifungal drug resistance.
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KEYWORD
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Cryptococcus neoformans, Nudix hydrolase, Oxidative stress, Ysa1
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