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KMID : 1160220140420010073
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Mycobiology
2014 Volume.42 No. 1 p.73 ~ p.78
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Molecular Epidemiology of Clinical Cryptococcus neoformans Isolates in Seoul, Korea
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Park So-Hae
Kim Mi-Na
Joo Sei-Ick
Hwang Soo-Myung
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Abstract
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Cryptococcal infection is primarily caused by two species, Cryptococcus neoformans and C. gattii. Between the twospecies, C. neoformans var. grubii is the major causative agent of cryptococcosis in Asia. We investigated the molecularcharacteristics of 46 isolates of C. neoformans from patients with cryptococcosis between 2008 and 2012 in Seoul, Korea. All theisolates were determined to be C. neoformans var. grubii (serotype A), mating type MATα, and molecular type VNI by PCRrestrictionfragment length polymorphism of the URA5 gene. Multilocus sequencing type (MLST) analysis using the InternationalSociety of Human and Animal Mycoses (ISHAM) consensus MLST scheme identified two sequence types (ST). Out of the 46strains, 44 (95.7%) were identified as ST5, and remaining 2 were identified as ST31. Our study revealed that the clinical strains ofC. neoformans in Korea are genetically homogeneous with the VNI/ST5 genotypes, and new appearance of VNI/ST31 genotypemay serve as an important indicator of global genetic analysis.
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KEYWORD
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Cryptococcus neoformans, Molecular type, Multilocus sequencing type
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