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KMID : 1160220140420020193
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Mycobiology
2014 Volume.42 No. 2 p.193 ~ p.197
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Overproduction of Laccase by the White-Rot Fungus Pleurotus ostreatus Using Apple Pomace as Inducer
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Park Young-Jin
Yoon Dae-Eun
Kim Hong-Il
Kwon O-chul
Yoo Young-Bok
Kong Won-Sik
Lee Chang-Soo
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Abstract
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Laccase activity of Pleurotus ostreatus is significantly increased by the addition of apple pomace. Among variousconditions, the best concentration of apple pomace and cultivation time for the production of laccase by P. ostreatus was 2.5%and 9 days, respectively. Reverse transcription polymerase chain reaction analyses of laccase isoenzyme genes, including pox1,pox3, pox4, poxc, poxa3, and poxa1b, revealed a clear effect of apple pomace on transcription induction. Our findings revealthat the use of apple pomace can be a model for the valuable addition of similar wastes and for the development of a solid-statefermenter and commercial production of oyster mushroom P. ostreatus.
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KEYWORD
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Apple pomace, Laccase, Pleurotus ostreatus
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