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KMID : 1160220140420030241
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Mycobiology
2014 Volume.42 No. 3 p.241 ~ p.248
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In Silico Sequence Analysis Reveals New Characteristics of Fungal NADPH Oxidase Genes
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Detry Nicolas
Choi Jae-Young
Kuo Hsiao-Che
Asiegbu Fred O.
Lee Yong-Hwan
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Abstract
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NADPH oxidases (Noxes), transmembrane proteins found in most eukaryotic species, generate reactive oxygen speciesand are thereby involved in essential biological processes. However, the fact that genes encoding ferric reductases and ferricchelatereductases share high sequence similarities and domains with Nox genes represents a challenge for bioinformaticapproaches used to identify Nox-encoding genes. Further, most studies on fungal Nox genes have focused mainly on functionality,rather than sequence properties, and consequently clear differentiation among the various Nox isoforms has not been achieved. We conducted an extensive sequence analysis to identify putative Nox genes among 34 eukaryotes, including 28 fungal genomesand one Oomycota genome. Analyses were performed with respect to phylogeny, transmembrane helices, di-histidine distance andglycosylation. Our analyses indicate that the sequence properties of fungal Nox genes are different from those of human and plantNox genes, thus providing novel insight that will enable more accurate identification and characterization of fungal Nox genes.
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KEYWORD
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Fungal genome, NADPH oxidase, Nox, Phylogenetics, ROS
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