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KMID : 1160220140420030249
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Mycobiology
2014 Volume.42 No. 3 p.249 ~ p.255
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Evaluating Carriers for Immobilizing Saccharomyces cerevisiae for Ethanol Production in a Continuous Column Reactor
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Cha Hye-Geun
Kim Yi-Ok
Choi Woon-Yong
Kang Do-Hyung
Lee Hyeon-Yong
Jung Kyung-Hwan
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Abstract
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We evaluated a more practical and cost-effective immobilization carriers for ethanol production using the yeastSaccharomyces cerevisiae. Three candidate materials?rice hull, rice straw, and sawdust?were tested for their cell-adsorptioncapacity and operational durability. Derivatizations of rice hull, rice straw, and sawdust with the optimal concentration of 0.5 M of2-(diethylamino)ethyl chloride hydrochloride (DEAE · HCl) resulted in > 95% adsorption of the initial yeast cells at 2 hr for DEAEricehull and DEAE-sawdust and in only approximately 80% adsorption for DEAE-rice straw. In addition, DEAE-sawdust was foundto be a more practical carrier for immobilizing yeast cells in terms of operational durability in shaking flask cultures with twodifferent speeds of 60 and 150 rpm. Furthermore, the biosorption isotherms of DEAE-rice hull, -rice straw, and -sawdust for yeastcells revealed that the Qmax of DEAE-sawdust (82.6 mg/g) was greater than that of DEAE-rice hull and DEAE-rice straw. During the404-hr of continuous column reactor operation using yeast cells immobilized on DEAE-sawdust, no serious detachment of theyeast cells from the DEAE-sawdust was recorded. Ethanol yield of approximately 3.04 g/L was produced steadily, and glucose wascompletely converted to ethanol at a yield of 0.375 g-ethanol/g-glucose (73.4% of the theoretical value). Thus, sawdust is apromising practical immobilization carrier for ethanol production, with significance in the production of bioethanol as a biofuel.
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KEYWORD
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Continuous column reactor, Ethanol production, Immobilization carrier, Saccharomyces cerevisiae, Sawdust
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