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KMID : 1160220140420030269
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Mycobiology
2014 Volume.42 No. 3 p.269 ~ p.273
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Identification of Acremonium acutatum and Trichothecium roseum isolated from Grape with White Stain Symptom in Korea
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Oh Soh-Young
Nam Ki-Woong
Yoon Deok-Hoon
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Abstract
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During 2010 and 2012 grape harvest seasons in Gyeonggi-do, Korea, a white stain symptom was observed on theharvested grape fruits in ‘Campbell-Early’ and ‘Kyoho’ varieties. In samples collected from the infected vine, two different strainsof pathogenic fungi have been found and identified as Acremonium acutatum and Trichothecium roseum based on fungalmorphology and nucleotide sequence of internal transcribed spacer (ITS) and supported by the phylogenetic analysis of therDNA-ITS region. The DNA homologies of the isolated strains were 99.8% and 99.6% identical with T. roseum (IFB-22133) andA. acutatum (CBS682.71), respectively. In the pathogenicity test, the spores of A. acutatum and T. roseum sprayed on the grapescaused white stain symptoms on the fruits in two weeks after the artificial inoculation, which is similar to observations in the field. To our knowledge, this is the first report of white stain symptoms caused by A. acutatum and T. roseum on the grapes in Korea
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KEYWORD
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Acremonium acutatum, Grape, Trichothecium roseum, White stain symptom
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