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KMID : 1160220140420040331
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Mycobiology
2014 Volume.42 No. 4 p.331 ~ p.338
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Effects of Water Stress on the Endophytic Fungal Communities of Pinus koraiensis Needles Infected by Cenangium ferruginosum
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Lee Sun-Keun
Lee Seung-Kyu
Bae Han-Hong
Seo Sang-Tae
Lee Jong-Kyu
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Abstract
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To examine the effects of water stress and Cenangium ferruginosum (CF) on the fungal endophytic community ofneedles of Pinus koraiensis (PK), fungal endophytes isolated from the needles of 5-year-old PK seedlings were compared beforeand after exposure to water stress conditions and artificial inoculation with CF ascospores. Artificial CF inoculation was successfullyconfirmed using PCR with CF-specific primers (CfF and CfR). For comparison of the degree of water deficit in water-stressed andcontrol groups of PK seedlings infected with CF, the water saturation deficit and water potential were measured. Lower waterpotential estimates were found in the water-stressed seedlings than in the control group. The fungal endophytes isolated from thesecond-year needles of non-water-stressed seedlings before and after CF inoculation revealed that primary saprobes wereapproximately 30% and 71.7%, respectively, and the remaining endophytes were rot fungi or pathogens. Sixty days after CFinoculation, diverse fungal endophytes in the first-year needles were isolated from the water-stressed seedlings. However, somefungal endophytes isolated from the non-water-stressed seedlings were also identified. Fungal endophytes in the second-yearneedles of the water-stressed and non-water-stressed seedlings were approximately 8% and 71.7% of saprobes, respectively, andthe remaining endophytes were rot fungi or pathogens. On the basis of the results, we conclude that water deficit and CF canhave an effect on fungal endophytic communities in the needles of PK seedlings.
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KEYWORD
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Cenangium ferruginosum, Fungal endophytic community, Pinus koriensis, Water stress
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