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KMID : 1160220140420040346
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Mycobiology
2014 Volume.42 No. 4 p.346 ~ p.352
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Influence of Additives on the Yield and Pathogenicity of Conidia Produced by Solid State Cultivation of an Isaria javanica Isolate
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Kim Jeong-Jun
Xie Ling
Han Ji-Hee
Lee Sang-Yeob
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Abstract
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Recently, the Q biotype of tobacco whitefly has been recognized as the most hazardous strain of Bemisia tabaciworldwide, because of its increased resistance to some insecticide groups. As an alternative control agent, we selected an Isariajavanica isolate as a candidate for the development of a mycopesticide against the Q biotype of sweet potato whitefly. To selectoptimal mass production media for solid-state fermentation, we compared the production yield and virulence of conidia between2 substrates (barley and brown rice), and we also compared the effects of various additives on conidia production and virulence. Barley was a better substrate for conidia production, producing 3.43 × 1010 conidia/g, compared with 3.05 × 1010 conidia/g forbrown rice. The addition of 2% CaCO3 + 2% CaSO4 to barley significantly increased conidia production. Addition of yeastextract, casein, or gluten also improved conidia production on barley. Gluten addition (3% and 1.32%) to brown rice improvedconidia production by 14 and 6 times, respectively, relative to brown rice without additives. Conidia cultivated on barleyproduced a mortality rate of 62% in the sweet potato whitefly after 4-day treatment, compared with 53% for conidia cultivatedon brown rice. The amendment of solid substrate cultivation with additives changed the virulence of the conidia produced; themedian lethal time (LT50) was shorter for conidia produced on barley and brown rice with added yeast extract (1.32% and 3%,respectively), KNO3 (0.6% and 1%), or gluten (1.32% and 3%) compared with conidia produced on substrates without additives.
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KEYWORD
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Bemisia tabaci, Biological control, Entomopathogenic fungi, Isaria javanica, Mass production, Solid-state fermentation
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