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KMID : 1160220150430020157
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Mycobiology
2015 Volume.43 No. 2 p.157 ~ p.162
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Optimization of Protein Extraction for Lichen Thalli
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Kondratiuk Anna S.
Savchuk Oleksiy M.
Hur Jae-Seoun
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Abstract
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Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study.
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KEYWORD
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Electrophoresis, Optimization method, Phenols, Proteins, Quinones
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