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KMID : 1160220170450040434
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Mycobiology
2017 Volume.45 No. 4 p.434 ~ p.437
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An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides
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Zhu Hanyu
Sun Xueyan
Liu Dongmei
Zheng Liesheng
Chen Liguo
Ma Aimin
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Abstract
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An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus? Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and 213 μg/g fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.
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KEYWORD
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Dimorphism, Jelly mushroom, RNA extraction, Tremella fuciformis
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