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KMID : 1160220180460010072
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Mycobiology
2018 Volume.46 No. 1 p.72 ~ p.78
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Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae
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Yao Fang-Jie
Lu Li-Xin
Wang Peng
Fanga Ming
Zhang You-Min
Chen Ying
Zhang Wei-Tong
Kong Xiang-Hui
Lu Jia
Honda Yoichi
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Abstract
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The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189?522?bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.
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KEYWORD
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Auricularia auricula-judae, fruiting body pattern, sequence-related amplified polymorphism, sequence characterized amplified region
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