|
KMID : 1160220190470040527
|
|
Mycobiology
2019 Volume.47 No. 4 p.527 ~ p.532
|
|
|
Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Agaricus bisporus)
|
|
An Hye-Jin
Jo Ick-Hyun
Oh Youn-Lee
Jang Kab-Yeul
Kong Won-Sik
Sung Jwa-Kyung
Chung Jong-Wook
|
|
|
Abstract
|
|
|
|
We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom (Agaricus bisporus), and selected 121 polymorphic markers. A total of 121 polymorphic markers, the average major allele frequency (MAF) and the average number of alleles (NA) were 0.50 and 5.47, respectively. The average number of genotypes (NG), observed heterozygosity (HO), expected heterozygosity (HE), and polymorphic information content (PIC) were 6.177, 0.227, 0.619, and 0.569, respectively. Pearson’s correlation coefficient showed that MAF was negatively correlated with NG (?0.683), NA (?0.600), HO (?0.584), and PIC (?0.941). NG, NA, HO, and PIC were positively correlated with other polymorphic parameters except for MAF. UPGMA clustering showed that 26?A. bisporus accessions were classified into 3 groups, and each accession was differentiated. The 121 SSR markers should facilitate the use of molecular markers in button mushroom breeding and genetic studies.
|
|
|
KEYWORD
|
|
|
Button mushroom, cluster analysis, genetic diversity, simple sequence repeat marker
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|