KMID : 1160220220500060467
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Mycobiology 2022 Volume.50 No. 6 p.467 ~ p.474
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The Autophagy Protein CsATG8 is Involved in Asexual Development and Virulence in the Pepper Anthracnose Fungus Colletotrichum scovillei
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Lee Kwang-Ho
Gumilang Adiyantara Fu Teng Kang Sung-Wook Kim Kyoung-Su
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Abstract
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Autophagy serves as a survival mechanism and plays important role in nutrient recycling under conditions of starvation, nutrient storage, ad differentiation of plant pathogenic fungi. However, autophagy-related genes have not been investigated in Colletotrichum scovillei, a causal agent of pepper fruit anthracnose disease. ATG8 is involved in autophagosome formation and is considered a marker of autophagy. Therefore, we generated an ATG8 deletion mutant, ¥ÄCsatg8, via homologous recombination to determine the functional roles of CsATG8 in the development and virulence of C. scovillei. Compared with the wild-type, the deletion mutant ¥ÄCsatg8 exhibited a severe reduction in conidiation. Conidia produced by ¥ÄCsatg8 were defective in survival, conidial germination, and appressorium formation. Moreover, conidia of ¥ÄCsatg8 showed reduced lipid amount and PTS1 selectivity. A virulence assay showed that anthracnose development on pepper fruits was reduced in ¥ÄCsatg8. Taken together, our results suggest that CsATG8 plays various roles in conidium production and associated development, and virulence in C. scovillei.
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KEYWORD
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Colletotrichum scovillei, pepper anthracnose, autophagy, CsATG8
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