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KMID : 1160220230510040230
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Mycobiology
2023 Volume.51 No. 4 p.230 ~ p.238
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Development and Optimization of Culture Medium for the Production of Glabridin by Aspergillus eucalypticola: An Endophytic Fungus Isolated from Glycyrrhiza glabra L. (Fabaceae)
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Parisa Bahadori Ganjabadi
Mohsen Farzaneh
Mohammad Hossein Mirjalili
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Abstract
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Glabridin is a well-known active isoflavone found in the root of licorice (Glycyrrhiza glabra L.)that possess a wide range of biological activity. Plant cells, hairy roots, and fungal endophytescultures are the most important alternative methods for plant resources conservationand sustainable production of natural compounds, which has received much attention inrecent decades. In the present study, an efficient culture condition was optimized for thebiomass accumulation and glabridin production from fungal endophyte Aspergillus eucalypticolaSBU-11AE isolated from licorice root. Type of culture medium, range of pH, and licoriceroot extract (as an elicitor) were tested. The results showed that the highest and lowestbiomass production was observed on PCB medium (6.43 ± 0.32 g/l) and peptone malt(5.85þ0.11 g/l), respectively. The medium culture PCB was produced the highest level ofglabridin (7.26 ± 0.44mg/l), while the lowest level (4.47 ± 0.02mg/l) was obtained from themedium peptone malt. The highest biomass (8.51 ± 0.43 g/l) and glabridin (8.30 ± 0.51mg/l)production were observed from the PCB medium adjusted with pH=6, while the lowestvalue of both traits was obtained from the same medium with pH=7. The highest productionof total glabridin (10.85 ± 0.84mg/l) was also obtained from the culture medium treatedwith 100 mg/l of the plant root extract. This information can be interestingly used for thecommercialization of glabridin production for further industrial applications.
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KEYWORD
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Endophyte, elicitation, flavone, licorice, pH, potato carrot broth
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