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KMID : 1160220230510040239
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Mycobiology
2023 Volume.51 No. 4 p.239 ~ p.245
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Isolation and Characterization of Xylanase from a Novel Strain, Penicillium menonorum SP10
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Thi Thu Huong Luong
Supattra Poeaim
Narumon Tangthirasunun
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Abstract
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Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles,and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi wereisolated, and their xylanase activities were tested by the qualitative method. The resultshowed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI)of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitativetest, isolate SP10 performed the highest xylanase activity with 0.535U/mL. Through molecularmethods using the b-tubulin gene, isolate SP10 was identified as Penicillium menonorum.
The xylanase characteristics from P. menonorum SP10 were determined, including the xylanaseisoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGEindicated that P. menonorum SP10 produced two xylanases (45 and 54 kDa). Moreover, itsxylanase worked optimally at pH 6 and 55 C while reaching 61% activity at 65 C. Theseresults proposed P. menonorum SP10 as a good candidate for industrial uses, especially inpoultry feed and pulp industries, to improve yield and economic efficiency under slightlyacidic and high-temperature conditions.
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KEYWORD
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Hemicellulose, Penicillium menonorum, xylanase, xylanase-producing fungi
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