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KMID : 1160619960010020214
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Preventive Nutrition and Food Science
1996 Volume.1 No. 2 p.214 ~ p.219
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Effects of Sardine Oil Feeding and Vitamin E Supplement on the Preneoplastic Hepatic Lesion and Cholesterol Metabolism in Hepatocarcinogenesis of Rats
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Kim Jung-Hee
Yoon Hye-Jin
Jang Ja-June
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Abstract
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This study was done to investigate effects of n-3 fatty acids and vitamin E supplement on the preneoplastic hepatic enzyme altered foci and cholesterol metabolism in experimental hepatocarcinogenesis system. Weaning male Sprague-Dawley rats were fed the diet containing either 15% corn oil(CO) or sardine oil(SO) with or without vitamin E 800IU supplementation for 12 weeks. After two weeks of feeding, rats were intraperitoneally injected with a single dose of diethylnitrosamine(DEN: 200㎎/㎏, BW). At the 4th week, rats were given the diet containing 0.02% acetylaminofluorene(AAF) for next 4 weeks. At the 6th week, 0.05% phenobarbital was incorporated into diet for 6 weeks. At the end of 12th week, rats were sacrificed and hepatic glutathione S-transferase placental form positive(GST-P^+) foci and serum and liver cholesterol levels were determined. GST-P^+ formation was significantly decreased by SO feeding when compared with CO feeding but it tended to be enhanced by vitamin E supplementation. Histopathological changes were similar to patterns of GST-P^+ formation in almost all dietary groups. Serum and hepatic cholesterol levels of SO fed groups were significantly lower than those of CO fed groups. Carcinogen treatments significantly increased serum and liver cholesterol levels in CO fed groups but not in SO fed groups. Correlation data showed a positive correlation(r=0.83, p＜0.01) between serum cholesterol level and GST-P^+ foci area. These results indicate that sardine oil as a n-3 fatty acid source may have a reducing effect on rat hepatocarcinogenesis by the alteration of cholesterol metabolism.
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KEYWORD
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P+ foci, sardine oil, vitamin E, cholesterol
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