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KMID : 1160619970020010061
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Preventive Nutrition and Food Science
1997 Volume.2 No. 1 p.61 ~ p.65
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Subcloning of Nodulin 26 Wild Type(S262) and Phosphorylation Site Mutant(S262D) into the Yeast Expression Vector pYES2
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Cha Youn-Soo
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Abstract
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Wild type nodulin 26(nod 26) cDNA(S262) and phodphorylation aite mutant(S262D) were constructed by a yeast expression system using pYES2 plasmids(pTES2-D262 and pTES2-S262D) were sc-reened by restriction mapping with BamHI of KpnI. S262 nod 26 contained a sreine residue at position 262 and S262D nod 26 contained the substitution mutation of serine to aspartic acid residue at position 262 were verified by automated floursent DNA sequencing.
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KEYWORD
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nodulin 26, plsmid, restriction enzyme, sequencing
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