KMID : 1160619970020030219
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Preventive Nutrition and Food Science 1997 Volume.2 No. 3 p.219 ~ p.224
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Molecular Size and Distribution of Zinc-binding Ligands in Rat Pancreatic Tissue
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Kwun In-Sook
Oberleas Donald
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Abstract
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The pancreas is an important organ in the maintenance of zinc homeostasis. The pancreatic tissue used in this study was obtained from rats fed varying levels of dietary Ca and phytate followed by intraperitoneal (65)^Zn injection. The objective of this study was to determine the molecular size and distribution of compounds that may represent zinc-binding complexes in pancreatic tissue homogenates. The supernatant of the homogenized pancreatic tissue was separated using a Sephadex G-75 column with Tris buffer at pH 8.1. All subfractions were assayed for zinc, protein and (65)^Zn activity. The elution of subfractions from pancreatic tissue homogenates showed a prominent peak corresponding to the high molecular weight protein standard (>66kd). A small molecular weigth protein (<6.5kd), that was absorbed at 280§¬, was also present; prominently in low Ca group, however not much as in high Ca group. These small compounds may combine weakly with zinc in pancreatic tissue and serve as zinc-binding ligands in pancreatic/biliary fluid. In the duodenum, these ligands dissociate zinc into an ionic form which becomes vulnerable to phytate complexation.
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KEYWORD
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rat pancreatic tissue homogenates, zinc-bindng ligands, endogenous zinc, zinc homeostasis
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