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KMID : 1160619980030020163
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Preventive Nutrition and Food Science
1998 Volume.3 No. 2 p.163 ~ p.168
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Effects of Indole Oligomers Induced from Indole-3-carbinol on the Growth of MCF-7 Breast Cancer Cells
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Kang Kap-Suk
Bjeldanes Leonard F.
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Abstract
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Inhibitory effect of indole oligomers induced from indole-3-carbinol(I3C) on the growth of breast cancer cells was studied. We gernerated the reaction mixtures (RXM) at ambient temperature by treating a stirred aqueous solution of I3C (typeically 0.25ml) at a concentration of 12 μmol/ml) with hydrochloric acid (typically 28μl of a 1 mmol/ml solution). RXM was fractionated by the column chromatography. The fractions with similar UV-pattern were further fractionated by HPLC and 3.3'-diindoylmethane (DIM) and other indole oligomers were identified. I3C, RXM, and it derived indole compounds were added to MCF-7 cells and cultured in the presence of 10-7M estradiol for 7 days. the growth-inhibitory effect of I3C and DIM on the growth of MCF-7 cell was very strong. The synthetic DIM also revealed antiproliferative effect on MCF-7 cel. The fractions containing high DIM content (77%), were most effective in inhibiting MCF-7 cell growth induced by estradiol. With these results, we suggest that I3C and DIM might have anticarcinogenic effect on the breast cancer.
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KEYWORD
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I3C, RXM, DIM, indole oligomer, anticarcinogen, cancer
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