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KMID : 1160619990040030167
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Preventive Nutrition and Food Science
1999 Volume.4 No. 3 p.167 ~ p.170
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Detectio of Malonaldehyde-thiobarbituric Acid (MA-TBA) Complex by High Performance Liquid Chromatography(HPLC) in a Model System
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Whang Key
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Abstract
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Various concentrations of malonaldehyde (MA) produced upon hydrolysis of 1, 1, 3,3-tetraethoxypropane (TEP) were reacted with 2-thiobarbituric acid (TBA)and th e contents of MA-TBA complex were measured both with spectrophotometer and high performance liquid chromatography (HPLC). As the concentrations of MA-TBA increased, their absorbances and the corresponding HPLC peak areas increased. The correlation coefficient between absorbances and HPLC peak areas of MA-TBA peaks from the other compounds and butanol extraction of the complex increased its recovery its recovery by 29.4% . Measurement of the content of MA-TBA complex for monitoring the development of lipid oxidation was proven to be successful with the use of high performance liquid chromatography.
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KEYWORD
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model system, high performance liquid chromatography, MA-TBA complex, ion pairing agent, butanol extraction
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